Carbohydratebindingspecificityofafucosespecificlectin. A typical lectin free diet excludes a range of foods, including most grains, pulses, and legumes, as well as eggs, seafood, and many staple fruits and vegetables. Relative intensities of recognition factors at two combining. In all cases, the quaternary structure is a tetramer arranged around a pseudo c222 axis of symmetry.
Another lectin that recognizes oligosaccharides containing core fucose would be a. The present communication reports the discovery of the second one, rs. Jul 29, 2005 the bacterium ralstonia solanacearum, which is distributed worldwide and causes lethal wilt in many agricultural crops, was shown to produce a potent l fucose binding lectin, r. We described previously the properties and sequence of the first lectin, rsl subunit mr 9. Six independent fucosebinding sites in the crystal structure of. With the exception of three lone proteins, these lectins can be classified into six families. The worldwide distributed plant aggressive pathogen ralstonia solanacearum, which causes lethal wilt in many agricultural crops, produces a potent lfucosebinding lectin rsl exhibiting sugar. Carbohydratebindingspecificityofafucosespecificlectin from. Plant pathogens, like animal ones, use proteincarbohydrate interactions in their strategy for host recognition, attachment, and invasion.
In this study, we report the heterologous expression and molecular binding properties of a fucosespecific lectin, aoflea, isolated from arthrobotrys oligospora. Files available from the acs website may be downloaded for personal use only. The fucosebinding lectin from ralstonia solanacearum. Fucosebinding lectin from opportunistic pathogen burkholderia. Many threedimensional structures of fucosebinding lectins have been reported. Preventing ralstonia solanacearum adhesion with glycans from. This is the first reported fucosespecific lectin found in nematophagous fungi.
The plant pathogen ralstonia solanacearum produces two lectins, each with different affinity to fucose. We compiled and compared the fucose binding lectins whose structures have been solved to date. Pdf the fucosebinding lectin from ralstonia solanacearum. Feb 03, 2012 analysis of the available genomes of b. Structures of the lectins from pseudomonas aeruginosa. Heterologous expression and molecular binding properties. In silico mutagenesis and docking study of ralstonia. Kostlanova n1, mitchell ep, lortatjacob h, oscarson s, lahmann m, gilboagarber n, chambat g, wimmerova m, imberty a. Fuc12gal containing oligosaccharides on certain plants 6.
Structural representation of rsl showing the r2ex data pdf. A new type of betapropeller architecture formed by oligomerization and interacting with fucoside, fucosyllactose, and plant xyloglucan. Anomerspecific recognition and dynamics in a fucosebinding. Rsl has a trimetric structure mw 30 kda composed of 90 amino acids 10. The bacterium ralstonia solanacearum, which is distributed worldwide and causes lethal wilt in many agricultural crops, was shown to produce a potent lfucosebinding lectin, r. Pdf production, properties and specificity of a new. Ralstonia solanacearum wilts many plants, causing heavy agricultural losses. Classification and comparison of fucosebinding lectins. Ralstonia solanacearum strain gmi pseudomonas solanacearum. Pdf fucosebinding lectin from opportunistic pathogen. During the past decades, the genome of this microbe relating to the molecular basis of its virulence has been revealed,, including its l fuc binding lectin.
In the present study, surface plasmon resonance experiments. The bacterium ralstonia solanacearum, which is distributed worldwide and causes lethal wilt in many agricultural crops, was shown to produce a potent l fucose binding lectin, r. The crystal structure of aol a fucosespecific lectin of aspergillus oryzae has. The trimeric solution structure and fucosebinding mechanism of the. Ralstonia solanacearum strain gmi pseudomonas solanacearum status. Aal 10, 11, aspergillus fumigatus afl 12, ralstonia solanacearum rsl, and.
Relative intensities of recognition factors at two. These lectins may bind to terminal lfucose, darabinose, and. Ralstonia solanacearum is one of the most destructive phytopathogenic bacteria on earth causing great agricultural and economical losses. Pdf anomerspecific recognition and dynamics in a fucose. Ralstonia solanacearum also produces a potent lfuctosebinding lectin, rsl, a small protein of 90 amino acids with a tandem repeat in its amino acid sequence. Three crystal structures of pail have been reported thus far. The lectin pail 51 kda, composed of four subunits of 121 amino acids, binds dgalactose plus its derivatives, and the lectin paiil 47 kda, composed of four subunits of 114 amino acids, binds lfucose and other monosaccharides.
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