Cells were sorted on high purity setting at no more than 5000 eventss using a 70 m nozzle at 55 psi with a 90 hz. Running a basic 2 color flow cytometry experiment in bd facs diva. User are asked to contact bd customer support center at 18772328995 if they have any additional questions. Facsdiva software the software uses a database server software to manage the flow cytometry data. These uniquely designed, gmp manufactured beads are excited by all currently supplied lasers and emit in the respective detectors for virtually any fluorescent dye.
Our selfpaced elearning courses can be used for basic learning or to refresh and reinforce what you learned in class. May 17, 20 bd biosciences has developed a protocol for enabling application settings on machines running the facsdiva v6 software, to create settings that allow for standardized consistent fluorescence intensity target values to be obtained across experiments run on different days or instruments. This manual describes how to use bd facsdiva software. If you are an experienced user, try the updated tutorials to. Access bd lsr ii online help from the help menu of bd facsdiva software.
Bd biosciences digital flow cytometry software provides. Bd facsdiva software resources software bd biosciences. Try free download manager fdm alternative software 1. Representative ctn data acquired on a bd facsverse system. In the login window, scroll down through the list of user names to locate yours. The bd facsdiva software reference manual assumes you have a working knowledge of basic microsoft windows operation. Regarding this runtime module, you are prohibited from. Includes training materials, snacks, coffee and lunch. Sophie novault bd facs diva software research institut. Computer and software login to log into the computer, use lsrii as the user name and lsrii as the password.
Cellquest is the data acquisition and analysis program from bd bioscience and operates in the macintosh environment on the facscalibur cytometers. This video describes the process of manual and auto compensation in bd facsdiva on the lsr ii. Dealing with a clog on the bd lsr ii flow cytometer duration. Aug 25, 2017 this video describes the process of manual and auto compensation in bd facsdiva on the lsr ii. Download popular programs, drivers and latest updates easily. Carleton stewart and rpci apoptosis prep for training. Dear all, a while ago there was a thread about how to recover deleted bd facsdiva files. Expand the specimen and make the tube active by clicking the arrow next to. For the demonstration, we will assume this is a 4color flow cytometric analysis. I only came upon this after our bd service engineer sent me a copy of it.
I had the same problem before and i hoped somebody would come up with a better solution than mine. For instructions on creating a user name and password, refer to the bd facsdiva software reference manual. The letter also stated that bd biosciences rep will contact the users when the new software is available. Since efficient imagestream data analysis demands ample computer storage space, powerful processing speed, and fast network connections, dedicated 64 bit. Refer to the information supplied by the manufacturer for applicationspecific limitations. Firsttime users of bd facsdiva software should read. If you are an experienced user, try the updated tutorials to become familiar with new software features. Along with builtin database and database utilities, bd facsdiva software provides automated data export in a variety of formats including xml, fcs, and even zip, for multiple files concurrently. History revision date change made 337647 rev a 0104 initial release for bd facsdiva software version 4.
To help customers take full and immediate advantage of their bd products capabilities, bd biosciences offers training for instruments, software, and applications. When autoplay is enabled, a suggested video will automatically play next. Bd facsdiva software is for in vitro diagnostic ivd use when used with ivd reagents and instruments. Feb 25, 20 how to use experiment layout in facsdiva raffaello cimbro. Bd facscanto clinical software can import worklists from bd facs sample prep assistant spa software v2. Upon startup, the software will connect to the cytometer. Use the following tutorials to learn how to use bd facsdiva software if you are a new user, or practice using new features if you are an. The runtime module was developed by a third party and we are obligated to notify our customers about the limitations for use. Bd facsdiva software provides one of the most complete and robust feature sets available for flow cytometry. The wide range of product courses includes handson, instructorled training, as well as elearning in instructor led virtual classrooms, selfpaced study, and online webinars. For the demonstration, we will assume this is a 4color flow cytometric analysis experiment. Winlist is a flow cytometry analysis software that operates in the pc windows environment. This version runs on the windows 7 32bit operating system.
Hts option for bd lsr ii 9 7 at the log in dialog, choose your user name, enter your password, and click ok. Running a basic 2 color flow cytometry experiment in bd. For the love of physics walter lewin may 16, 2011 duration. Use the following tutorials to learn how to use bd facsdiva software if you are a new user, or practice using new features if you are an experienced user. Lsr iib and lsriic turn on the fluidics pump and ensure that it is running. All data, plots, gates, instrument settings, and statistics are saved inside the database. Bd facsmelody online training program additional content available in the elearning library. Ddx4positive and negative cells, for both human ovarian cells and transfected hek 293 t cells, were sorted using an aria iiu cytometer equipped with a 488 nm laser, running bd facsdiva v6 software bd biosciences, oxford, uk. Refilling sheath tank and emptying waste on bd lsr ii flow cytometer. Bd facsdiva software overview selfpaced data display in bd facsdiva software selfpaced using the worksheet window in bd facsdiva software selfpaced using templates in bd facsdiva software selfpaced bd facsvia online training program. The bd lsr ii is the first and only aircooled, fourlaser benchtop flow cytometer with the ability to acquire up to 18 colors. To log in to the facsdiva software you need to locate the icon on the desktop and double click it. A forward scatter vs side scatter of ctn using bd facsuite software b plot showing aggregates on the basis of propidium iodide width w vs propidium iodide area a c resolution and linearity shown by a propidium iodide area histogram of propidium iodide stained ctn. Bd facsdiva software, free download by becton, dickinson and company.
Interference there are no known incompatibilities with bd facscanto clinical. The format of the user name for facsdiva software is the first letter of your first name followed by your full last name. Bd facsdiva software reference manual for version 8. The lsrii, fortessa, x20, and symphony appear different, but the operation and workflow are performed in the same manner on all instrument types. Bd biosceinces instrument and application trainings.
Bd facsdiva software features organization bd facsdiva. Bd facsdiva software reference manual for version 7. Download bd facsdiva software by becton, dickinson and company. Bd facsdiva software features bd facsdiva software bd. Welcome to the introduction to bd facslink lis interface solution with bd facsdiva software webbased training. See using the worksheet grid tab on page 186 in the bd facsdiva software reference manual. If you are not familiar with the windows operating system, see the documentation provided with your computer. Other software fcs and setup files created within bd facscanto clinical software can be imported into bd facsdiva software v5. Compensation of a 7 color panel on the bd lsr ii uc merced scif stem cell instrumentation foundry. Compensation of a 7 color panel on the bd lsr ii youtube. Carleton stewart and rpci titering directly conjugated antibodies to extracellular antigens special thanks to dr. Bd facsdiva software contains vxworks as embedded software runtime module.
The first, oddly enough, is bds very own index sorting manual tutorial. Bd compbeads plus bd facsdiva software pdf bd facsaria users guide. Facsdiva is bd biosciences next generation data acquisition and analysis program and operates in the pc windows environment on the facsaria, facscanto, lsrii and fortessa. For information on how to operate and maintain your flow cytometer, see your cytometer manual. Bd biosciences will release a version of bd facsdiva software to correct this problem. Bd facsdiva software is for in vitro diagnostic ivd use when used with ivd reagents and cytometers. Here well have a discussion covering many topics relating to the bd facsdiva platform including software and hardware. Dec 30, 2017 this video will show you how to install bd facsdiva 8. Running a basic 2 color flow cytometry experiment in bd facs. Next you want to tell the software what populations to show in each plot. The bd facsdiva software quick start guide contains tutorials designed to familiarize you with bd facsdiva software. The user cannot specify any path for saving, everything is done automatically in. Bd lsr ii facility requirement guide contains specifications for.
Index sorting from facsdiva to flowjo flow cytometry news. Analysis software roswell park comprehensive cancer center. To help customers, take full and immediate advantage of their bd products capabilities, bd biosciences offers training for instruments, software, and applications. Verify that the breakoff is stable and turn on the sweet spot. This video describes how to set up an experiment in facs diva version 8. Using templates in bd facsdiva software selfpaced bd facsvia online training program. Mar 01, 2015 the first, oddly enough, is bd s very own index sorting manual facsdiva software manual and may not even be installed on your computer or available for download from bd s website. Bd facsdiva software v6 with sample preppers bd facsdiva software v6 with spa ii and lwa sample preppers bd facsdiva software v6 with spa iii and lwa sample.
At the heart of the bd lsr ii is the new digital acquisition, allowing for additional parameters to be detected, and yielding more information per cell. If you are a new user, use the tutorials to get started with the software. Bd facsdiva software is a collection of rich tools for flow cytometer and application setup, data acquisition, and data analysis that help streamline flow cytometry workflows for todays busy laboratory. Cellquest acquistion tutorial cellquest analysis tutorial cellquest users guide vendor website facsdiva is bd biosciences next generation data acquisition and analysis program and operates in the pc windows environment on the facsaria, facscanto. If you are not familiar with the windows operating system, refer to the documentation provided with your computer. After a successful login, the bd facsdiva workspace appears showing the main application windows which include browser, cytometer, inspector, acquistion dashboard and global worksheet. For information on how to operate and maintain your flow cytometer, refer to the instrument users.
Apr 03, 2014 here well have a discussion covering many topics relating to the bd facsdiva platform including software and hardware. Developed in collaboration with data innovations, a leading middleware provider experienced in clinical applications, this laboratory information system lis interface solution allows the direct transfer of information between bd software and an lis, reducing errors commonly caused. Completion of a bd operator course for a digital cytometer using bd facsdiva software v6 or equivalent experience on a bd digital cytometer. This guide contains instructions for using bd facsdiva software version 6. Proceed to the website of becton, dickinson and company. Bd facsdiva software basic experiment guide please note, this guide is not intended to take the place of inperson training but is to be used as a reference as needed after completing training with unified flow core staff. See headers and footers on page 100 in the bd facsdiva software reference manual. Turn on the instrument using the green power button. The most popular version of this product among our users is 4.
Allow the instrument to connect to the software if necessary. To log into the computer, use lsrii as the user name and lsrii as the password. Worksheets are cause effect, bd lsrii overview workbook, bd cytometric bead array software users guide, getting started with bd facsdiva software, bd facsdiva software reference manual, chapters 1, practice with all s t be sure to state, bd facsdiva tutorial. The name of the program executable file is bd facsdiva software. Bd facsdiva software offers efficient data management tools to simplify data storage as well as improve organization of flow cytometer data. This guide contains instructions for using bd facsdiva. Aug 17, 2017 running a basic 2 color flow cytometry experiment in bd facs diva uc merced scif stem cell instrumentation foundry. Bd cytometer setup and tracking application guide cytometer configuration setup bd facsdiva.
1019 1273 444 680 1177 626 485 395 721 633 341 1337 155 716 499 333 361 1141 1048 201 159 672 1050 746 1142 817 1120 666 143 1287 965